ABSTRACT
AIM To establish an HPLC method for the simultaneous content determination of catalpol,acteoside,jionoside B1,martynoside,lobetyolin,methylophiopogonanone A and methylophiopogonanone B in Dangshen Guben Pills (Rehmanniae Radix,Rehmanniae Radix Preparata,Codonopsis Radix,etc.).METHODS The analysis of 50％ methanol extract of this drug was performed on a 30 ℃C thermostatic Agilent TC-C1s column (200 mm ×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.2％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 210,330,269 and 296 nm.RESULTS Seven constituents showed good linear relationships within their own ranges (r ≥0.999 2),whose average recoveries were 96.96％-99.65％ with the RSDs of 0.88％-1.74％.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Dangshen Guben Pills.
ABSTRACT
AIM To establish an HPLC method for the simultaneous content determination of catalpol,acteoside,jionoside B1,martynoside,lobetyolin,methylophiopogonanone A and methylophiopogonanone B in Dangshen Guben Pills (Rehmanniae Radix,Rehmanniae Radix Preparata,Codonopsis Radix,etc.).METHODS The analysis of 50％ methanol extract of this drug was performed on a 30 ℃C thermostatic Agilent TC-C1s column (200 mm ×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.2％ phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 210,330,269 and 296 nm.RESULTS Seven constituents showed good linear relationships within their own ranges (r ≥0.999 2),whose average recoveries were 96.96％-99.65％ with the RSDs of 0.88％-1.74％.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Dangshen Guben Pills.